Scanning electron microscopy as a method for sample visualization in protein X-ray crystallography.

ABSTRACT: Developing methods to determine high-resolution structures from micrometre- or even submicrometre-sized protein crystals has become increasingly important in recent years. This applies to both large protein complexes and membrane proteins, where protein production and the subsequent growth of large homogeneous crystals is often challenging, and to samples which yield only micro- or nanocrystals such as amyloid or viral polyhedrin proteins. The versatile macromolecular crystallography microfocus (VMXm) beamline at Diamond Light Source specializes in X-ray diffraction measurements from micro- and nanocrystals. Because of the possibility of measuring data from crystalline samples that approach the resolution limit of visible-light microscopy, the beamline design includes a scanning electron m

SUBMITTER: Beale EV 

PROVIDER: S-EPMC7201292 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature