Identification of the distribution of human endogenous retroviruses K (HML-2) by PCR-based target enrichment sequencing.

ABSTRACT:

Background

Human endogenous retroviruses (HERVs), suspected to be transposition-defective, may reshape the transcriptional network of the human genome by regulatory elements distributed in their long terminal repeats (LTRs). HERV-K (HML-2), the most preserved group with the least number of accumulated of mutations, has been associated with aberrant gene expression in tumorigenesis and autoimmune diseases. Because of the high sequence similarity between different HERV-Ks, current methods have limitations in providing genome-wide mapping specific for individual HERV-K (HML-2) members, a major barrier in delineating HERV-K (HML-2) function.

Results

In an attempt to obtain detailed distribution information of HERV-K (HML-2), we utilized a PCR-based target enrichment sequencing p

SUBMITTER: Xue B 

PROVIDER: S-EPMC7201656 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature