Dataset Information

Anchor Away - A Fast, Reliable and Reversible Technique To Inhibit Proteins in Drosophila melanogaster.

ABSTRACT: Several techniques have been developed to study specific gene function in loss-of-function situations. In Drosophila melanoga st er, RNAi and the generation of mutant clones are widely used. However, both techniques have the limitation that there is a significant time lag before gene function is abolished. Given the relatively rapid development of Drosophila, such perdurance is a serious impediment to study gene function. Here we describe the adaptation of the anchor-away technique for use in Drosophila Anchor-away was originally developed in yeast to quickly and efficiently abrogate the function of nuclear proteins by sequestering - anchoring - them away in a different cellular compartment. The required components are present in the cells, and the system is triggered by the addition of rapamycin, resulting in a rapid generation of a loss-of-function situation. We provide here proof of principle for the system by producing loss-of-function situations for two nuclear proteins - Pygopus and Brinker. The system allows to study the requirement of any protein during any time window, and at the same time circumvents difficulties, such as off-target effects or variable phenotypes, which are inherent in other techniques, for example RNAi.

SUBMITTER: Bosch PS

PROVIDER: S-EPMC7202031 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Anchor Away - A Fast, Reliable and Reversible Technique To Inhibit Proteins in Drosophila melanogaster.

Bosch Pablo Sanchez PS Pepperl Julia J Basler Konrad K

G3 (Bethesda, Md.) 20200504 5

Several techniques have been developed to study specific gene function in loss-of-function situations. In Drosophila melanogaster, RNAi and the generation of mutant clones are widely used. However, both techniques have the limitation that there is a significant time lag before gene function is abolished. Given the relatively rapid development of Drosophila, such perdurance is a serious impediment to study gene function. Here we describe the adaptation of the anchor-aw ...[more]

PMID: 32217630

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Dataset Information

Anchor Away - A Fast, Reliable and Reversible Technique To Inhibit Proteins in Drosophila melanogaster.

Publications

Anchor Away - A Fast, Reliable and Reversible Technique To Inhibit Proteins in <i>Drosophila melanogaster</i>.

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