Image-derived models of cell organization changes during differentiation and drug treatments.

ABSTRACT: PC12 cells are a popular model system to study changes driving and accompanying neuronal differentiation. While attention has been paid to changes in transcriptional regulation and protein signaling, much less is known about the changes in organization that accompany PC12 differentiation. Fluorescence microscopy can provide extensive information about these changes, although it is difficult to continuously observe changes over many days of differentiation. We describe a generative model of differentiation-associated changes in cell and nuclear shape and their relationship to mitochondrial distribution constructed from images of different cells at discrete time points. We show that the model accurately represents complex cell and nuclear shapes and learn a regression model that relates cell

SUBMITTER: Ruan X 

PROVIDER: S-EPMC7202072 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature