MicroRNA-375 exacerbates knee osteoarthritis through repressing chondrocyte autophagy by targeting ATG2B.

ABSTRACT:

Objective

This study aimed to explore the underlying mechanism of miR-375 in exacerbating osteoarthritis (OA).

Results

MiR-375 expression were upregulated in OA cartilage tissues, whereas ATG2B expression was decreased. MiR-375 targeted ATG2B 3' UTR and inhibited its expression in the chondrocytes, and then suppressed autophagy and promoted endoplasmic reticulum stress (ERs). The apoptosis rate of chondrocytes was increased after being transfected with miR-375 mimics. In vivo results further verified that inhibition of miR-375 could relieve OA-related symptoms.

Conclusion

miR-375 can inhibit the expression of ATG2B in chondrocytes, suppress autophagy and promote the ERs. It suggests that miR-375 could be considered to be a key therapy target for OA.

Methods<

SUBMITTER: Li H 

PROVIDER: S-EPMC7202526 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature