Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration.

ABSTRACT: Cells change migration patterns in response to chemical stimuli, including the gradients of the stimuli. Cellular migration in the direction of a chemical gradient, known as chemotaxis, plays an important role in development, the immune response, wound healing, and cancer metastasis. While chemotaxis modulates the migration of single cells as well as collections of cells in vivo, in vitro research focuses on single-cell chemotaxis, partly due to the lack of the proper experimental tools. To fill that gap, described here is a unique experimental system that combines microfluidics and micropatterning to demonstrate the effects of chemical gradients on collective cell migration. Furthermore, traction microscopy and monolayer stress microscopy are incorporated into the system to characterize c

SUBMITTER: Jang H 

PROVIDER: S-EPMC7202863 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature