Microrheology for Hi-C Data Reveals the Spectrum of the Dynamic 3D Genome Organization.

ABSTRACT: The one-dimensional information of genomic DNA is hierarchically packed inside the eukaryotic cell nucleus and organized in a three-dimensional (3D) space. Genome-wide chromosome conformation capture (Hi-C) methods have uncovered the 3D genome organization and revealed multiscale chromatin domains of compartments and topologically associating domains (TADs). Moreover, single-nucleosome live-cell imaging experiments have revealed the dynamic organization of chromatin domains caused by stochastic thermal fluctuations. However, the mechanism underlying the dynamic regulation of such hierarchical and structural chromatin units within the microscale thermal medium remains unclear. Microrheology is a way to measure dynamic viscoelastic properties coupling between thermal microenvironment and mec

SUBMITTER: Shinkai S 

PROVIDER: S-EPMC7203008 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature