Resistance of Klebsiella pneumoniae Strains Carrying bla _NDM-1 Gene and the Genetic Environment of bla _NDM-1.

ABSTRACT:

Objective

Regional dissemination is the major cause of the widespread prevalence of a plasmid-encoding NDM-1 enzyme. We investigated the drug resistance, joint efficiency, and gene environment of a Klebsiella pneumoniae strain carrying bla _NDM-1 gene.

Materials and methods

Carbapenem-non-susceptible strains were analyzed using the VITEK 2 Compact. Strains carrying bla _NDM-1 were identified using polymerase chain reaction and sequencing. Antimicrobial susceptibility testing and plasmid conjugation experiments were then conducted. Strains carrying bla _NDM-1 were subjected to Southern blot analysis. After the gene mapping of bla _NDM-1, library construction, and sequencing, plasmids were subsequently

SUBMITTER: Xiang T 

PROVIDER: S-EPMC7203411 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature