Rapid online buffer exchange for screening of proteins, protein complexes and cell lysates by native mass spectrometry.

ABSTRACT: It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as for use in consumer products, chemical processes and therapeutics. Native mass spectrometry (nMS) has become an important tool in protein analysis due to its ability to retain non-covalent interactions during measurements, making it possible to obtain protein structural information with high sensitivity and at high speed. Interferences from the presence of non-volatiles are typically alleviated by offline buffer exchange, which is time-consuming and difficult to automate. We provide a protocol for rapid online buffer exchange (OBE) nMS to directly screen

SUBMITTER: VanAernum ZL 

PROVIDER: S-EPMC7203678 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature