Generalizable sgRNA design for improved CRISPR/Cas9 editing efficiency.

ABSTRACT:

Motivation

The development of clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) technology has provided a simple yet powerful system for targeted genome editing. In recent years, this system has been widely used for various gene editing applications. The CRISPR editing efficacy is mainly dependent on the single guide RNA (sgRNA), which guides Cas9 for genome cleavage. While there have been multiple attempts at improving sgRNA design, there is a pressing need for greater sgRNA potency and generalizability across various experimental conditions.

Results

We employed a unique plasmid library expressed in human cells to quantify the potency of thousands of CRISPR/Cas9 sgRNAs. Differential sequence and structural features among th

SUBMITTER: Hiranniramol K 

PROVIDER: S-EPMC7203743 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature