Comprehensive dissection of dispensable genomic regions in Streptomyces based on comparative analysis approach.

ABSTRACT: BACKGROUND:Large-scale genome reduction has been performed to significantly improve the performance of microbial chassis. Identification of the essential or dispensable genes is pivotal for genome reduction to avoid synthetic lethality. Here, taking Streptomyces as an example, we developed a combinatorial strategy for systematic identification of large and dispensable genomic regions in Streptomyces based on multi-omics approaches. RESULTS:Phylogenetic tree analysis revealed that the model strains including S. coelicolor A3(2), S. albus J1074 and S. avermitilis MA-4680 were preferred reference for comparative analysis of candidate genomes. Multiple genome alignment suggested that the Streptomyces genomes embodied highly conserved core region and variable sub-telomeric regions, and may pres

SUBMITTER: Bu QT 

PROVIDER: S-EPMC7204314 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature