TnFLX: a Third-Generation mariner-Based Transposon System for Bacillus subtilis.

ABSTRACT: Random transposon mutagenesis is a powerful and unbiased genetic approach to answer fundamental biological questions. Here, we introduce an improved mariner-based transposon system with enhanced stability during propagation and versatile applications in mutagenesis. We used a low-copy-number plasmid as a transposon delivery vehicle, which affords a lower frequency of unintended recombination during vector construction and propagation in Escherichia coli We generated a variety of transposons allowing for gene disruption or artificial overexpression, each in combination with one of four different antibiotic resistance markers. In addition, we provide transposons that will report gene/protein expression due to transcriptional or translational coupling. We believe that the TnFLX system will he

SUBMITTER: Dempwolff F 

PROVIDER: S-EPMC7205501 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature