LGR5⁺ epithelial tumor stem-like cells generate a 3D-organoid model for ameloblastoma.

ABSTRACT: Ameloblastoma (AM) is a benign but locally aggressive tumor with high recurrences. Currently, underlying pathophysiology remains elusive, and radical surgery remains the most definitive treatment with severe morbidities. We have recently reported that AM harbors a subpopulation of tumor epithelial stem-like cells (AM-EpiSCs). Herein, we explored whether LGR5⁺ epithelial cells in AM possess stem-like cell properties and their potential contribution to pathogenesis and recurrence of AM. We found that LGR5 and stem cell-related genes were co-expressed in a subpopulation of AM epithelial cells both in vivo and in vitro, which were enriched under 3D-spheroid culture. As compared to LGR5^- counterparts, LGR5⁺ AM epithelial cells showed increased expression of vari

SUBMITTER: Chang TH 

PROVIDER: S-EPMC7206107 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature