Effect of all-trans retinoic acid on casein and fatty acid synthesis in MAC-T cells.

ABSTRACT: OBJECTIVE:Caseins and fatty acids of milk are synthesized and secreted by the epithelial cells of the mammary gland. All-trans retinoic acid (ATRA), an active metabolite of vitamin A, has been shown to promote mammary development. This study was conducted to determine the effect of ATRA on casein synthesis and fatty acid composition in MAC-T cells. METHODS:MAC-T cells were allowed to differentiate for 4 d, treated with ATRA (0, 1.0, 1.5, and 2.0 μM), and incubated for 3 d. We analyzed the fatty acid composition, the mRNA expression of casein and fatty acid synthesis-related genes, and the phosphorylation of casein synthesis-related proteins of MAC-T cells by gas chromatography, quantitative polymerase chain reaction, and western blotting, respectively. RESULTS:In MAC-T cells, ATRA increase

SUBMITTER: Liao XD 

PROVIDER: S-EPMC7206372 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature