Temporal expression profiling of long noncoding RNA and mRNA in the peripheral blood during porcine development.

ABSTRACT:

Objective

We investigated the temporal expression profiles of long noncoding RNA (lncRNA) and mRNA in the peripheral blood of pigs during development and identified the lncRNAs that are related to the blood-based immune system.

Methods

Peripheral blood samples were obtained from the pigs at 0, 7, 28, and 180 days and 2 years of age. RNA sequencing was performed to survey the lncRNA and mRNA transcriptomes in the samples. Short time-series expression miner (STEM) was used to show temporal expression patterns in the mRNAs and lncRNAs. Gene ontology and Kyoto encyclopedia of genes and genomes analyses were performed to assess the genes' biological relevance. To predict the functions of the identified lncRNAs, we extracted mRNAs that were nearby loci and highly correlated with t

SUBMITTER: Gu Y 

PROVIDER: S-EPMC7206404 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature