Characterization of a novel Mycoplasma cynos real-time PCR assay.

ABSTRACT: Mycoplasma cynos is recognized as an emerging causative pathogen of canine infectious respiratory disease (CIRD) worldwide. We developed a new open-source real-time PCR (rtPCR) assay for M. cynos that performs well under standard rtPCR conditions. Primers and probes were designed to target the M. cynos tuf gene. Reaction efficiencies for the M. cynos tuf gene assay on 2 platforms were based on amplification of standard curves spanning 8 orders of magnitude: ABI 7500 platform, 94.3-97.9% (r² ≥ 0.9935); QuantStudio OpenArray platform, 119.1-122.5% (r² = 0.9784). The assay performed very well over a range of template input, from 10⁹ copies to the lower limit of quantification at 4 copies of the M. cynos genome o

SUBMITTER: Tallmadge RL 

PROVIDER: S-EPMC7206559 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature