Negative depletion mediated brightfield circulating tumour cell identification strategy on microparticle-based microfluidic chip.

ABSTRACT:

Background

The most convenient circulating tumor cells (CTCs) identification method is direct analysis of cells under bright field microscopy by which CTCs can be comprehensive studied based on morphology, phenotype or even cellular function. However, universal cell markers and a standard tumour cell map do not exist, thus limiting the clinical application of CTCs.

Results

This paper focuses on an automatic and convenient negative depletion strategy for circulating tumour cell identification under bright field microscopy. In this strategy, immune microparticles (IMPs) are applied to negatively label white blood cells rather than the tumour cells, such that tumour cells can be directly distinguished under brightfield of the microscopy. In this way, all of the heterogeneous tu

SUBMITTER: Wang S 

PROVIDER: S-EPMC7206695 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature