BK_Ca Mediates Dysfunction in High Glucose Induced Mesangial Cell Injury via TGF-β1/Smad2/3 Signaling Pathways.

ABSTRACT:

Objective

To explore the role and mechanism of BK_Ca in diabetic kidney disease.

Methods

Rat mesangial cells (MCs) HBZY-1 were cultured with high glucose to simulate the high-glucose environment of diabetic kidney disease in vivo. The effects of large conductance calcium-activated potassium channel (BK_Ca) on proliferation, migration, and apoptosis of HBZY-1 cells were observed. The contents of transforming growth factor beta 1 (TGF-β1), Smad2/3, collagen IV (Col IV), and fibronectin (FN) in the extracellular matrix were also observed.

Results

High glucose significantly damaged HBZY-1 cells, which enhanced the ability of cell proliferation, migration, and apoptosis, and increased the secretion of Col IV and FN. Inhibition of BK_Ca

SUBMITTER: Wu Z 

PROVIDER: S-EPMC7206888 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature