Overexpression of a developing xylem cDNA library in transgenic poplar generates high mutation rate specific to wood formation.

ABSTRACT: We investigated feasibility of the Full-length complementary DNA OvereXpression (FOX) system as a mutagenesis approach in poplar, using developing xylem tissue. The main goal was to assess the overall mutation rate and if the system will increase instances of mutants affected in traits linked to the xylem tissue. Indeed, we found a high mutation rate of 17.7%, whereas 80% of all mutants were significantly affected in cellulose, lignin and/or hemicellulose. Cell wall biosynthesis is a major process occurring during xylem development. Enrichment of mutants affected in cell wall composition suggests that the tissue source for the FOX library influenced the occurrence of mutants affected in a trait linked to this tissue. Additionally, we found that FLcDNAs from mutants affected in cell wall co

SUBMITTER: Rauschendorfer J 

PROVIDER: S-EPMC7207001 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature