Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection.

ABSTRACT: The phenotypic and functional dichotomy between IRF8⁺ type 1 and IRF4⁺ type 2 conventional dendritic cells (cDC1s and cDC2s, respectively) is well accepted; it is unknown how robust this dichotomy is under inflammatory conditions, when additionally monocyte-derived cells (MCs) become competent antigen-presenting cells (APCs). Using single-cell technologies in models of respiratory viral infection, we found that lung cDC2s acquired expression of the Fc receptor CD64 shared with MCs and of IRF8 shared with cDC1s. These inflammatory cDC2s (inf-cDC2s) were superior in inducing CD4⁺ T helper (Th) cell polarization while simultaneously presenting antigen to CD8⁺ T cells. When carefully separated from inf-cDC2s, MCs lacked APC function. Inf-cDC2s mature

SUBMITTER: Bosteels C 

PROVIDER: S-EPMC7207120 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature