STATR: A simple analysis pipeline of Ribo-Seq in bacteria.

ABSTRACT: Gene expression changes in response to diverse environmental stimuli to regulate numerous cellular functions. Genes are expressed into their functional products with the help of messenger RNA (mRNA). Thus, measuring levels of mRNA in cells is important to understand cellular functions. With advances in next-generation sequencing (NGS), the abundance of cellular mRNA has been elucidated via transcriptome sequencing. However, several studies have found a discrepancy between mRNA abundance and protein levels induced by translational regulation, including different rates of ribosome entry and translational pausing. As such, the levels of mRNA are not necessarily a direct representation of the protein levels found in a cell. To determine a more precise way to measure protein expression in cells

SUBMITTER: Choe D 

PROVIDER: S-EPMC7209825 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature