Mechanical regulation of glycolysis via cytoskeleton architecture.

ABSTRACT: The mechanics of the cellular microenvironment continuously modulates cell functions such as growth, survival, apoptosis, differentiation and morphogenesis via cytoskeletal remodelling and actomyosin contractility^1-3. Although all of these processes consume energy^4,5, it is unknown whether and how cells adapt their metabolic activity to variable mechanical cues. Here we report that the transfer of human bronchial epithelial cells from stiff to soft substrates causes a downregulation of glycolysis via proteasomal degradation of the rate-limiting metabolic enzyme phosphofructokinase (PFK). PFK degradation is triggered by the disassembly of stress fibres, which releases the PFK-targeting E3 ubiquitin ligase tripartite motif (TRIM)-containing protein 21 (TRIM21). Transfor

SUBMITTER: Park JS 

PROVIDER: S-EPMC7210009 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature