Sam68 promotes aerobic glycolysis in colorectal cancer by regulating PKM2 alternative splicing.

ABSTRACT:

Background

Sam68, an RNA-binding protein, exerts oncogenic functions in several types of cancer. However, the specific functions and mechanisms of Sam68 in colorectal cancer (CRC) had not been previously clarified. Pyruvate kinase muscle (PKM)2 is the key rate-limiting enzyme in glycolysis, and PKM2 maintains the glycolysis-dominant energy metabolism in most cancer cells.

Methods

CCK8 assay was performed to show the effect of Sam68 on cell growth. Pyruvate kinase activity and lactate detection assays were performed to analyze the effects of Sam68 on aerobic glycolysis. RNA immunoprecipitation (RIP) was used to detect the binding of Sam68 to the PKM2 sequence. Western blot and real-time PCR were executed to analyze the regulation of PKM2 by Sam68.

Results

SUBMITTER: Zhao J 

PROVIDER: S-EPMC7210197 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature