Cell adhesion molecule 2 (CADM2) promotes brain metastasis by inducing epithelial-mesenchymal transition (EMT) in human non-small cell lung cancer.

ABSTRACT:

Background

To investigate the effect of CADM2 on brain metastasis in non-small cell lung cancer (NSCLC).

Methods

Human transcriptome-wide microarray analysis was used to identify gene expression in lung tissue of NSCLC patients with or without brain metastasis, which indicated that CADM2 was significantly up-regulated. Quantitative real-time PCR (qRT-PCR) was used to confirm the CADM2 up-regulation further. SiRNA was used to knock down the expression of CADM2 in NSCLC cell lines and a Transwell assay was performed to determine the effects of CADM2 knockdown on cell migration and invasion. The expressions of Vimentin and E-cadherin were detected by western blot assay.

Results

The result of microarray analysis and qRT-PCR showed that CADM2 was significantly up-regulated

SUBMITTER: Dai L 

PROVIDER: S-EPMC7210202 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature