Sero-epidemiological study of the rotavirus VP8* protein from different P genotypes in Valencia, Spain.

ABSTRACT: The aims of the present work were to determine the prevalence and titer of serum antibodies against several rotavirus VP8* proteins from different P genotypes in children and adults in Valencia, Spain; and to determine the role of the secretor status (FUT2_G428A polymorphism) in the antibody response. The VP8* protein from the P[4], P[6], P[8], P[9], P[11], P[14] and P[25] genotypes were produced in E. coli. These proteins were tested with 88 serum samples from children (n = 41, 3.5 years old in average) and from adults (n = 47, 58 years old in average) by ELISA. A subset of 55 samples were genotyped for the FUT2_G428A polymorphism and the antibody titers compared. The same subset of samples was also analysed by ELISA using whole rotavirus Wa particles (G1P[8]) as antig

SUBMITTER: Vila-Vicent S 

PROVIDER: S-EPMC7210269 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature