Light-powered Escherichia coli cell division for chemical production.

ABSTRACT: Cell division can perturb the metabolic performance of industrial microbes. The C period of cell division starts from the initiation to the termination of DNA replication, whereas the D period is the bacterial division process. Here, we first shorten the C and D periods of E. coli by controlling the expression of the ribonucleotide reductase NrdAB and division proteins FtsZA through blue light and near-infrared light activation, respectively. It increases the specific surface area to 3.7 μm^-1 and acetoin titer to 67.2 g·L^-1. Next, we prolong the C and D periods of E. coli by regulating the expression of the ribonucleotide reductase NrdA and division protein inhibitor SulA through blue light activation-repression and near-infrared (NIR) light activation, respectively.

SUBMITTER: Ding Q 

PROVIDER: S-EPMC7210317 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature