Identification of Bupleurum (Apiaceae) seeds by allele-specific PCR based on ITS sequences.

ABSTRACT: The traditional Chinese medicine Bupleuri radix (chaihu) is the dried roots of Bupleurum chinense and Bupleurum scorzonerifolium and many adulterants exist because of the differences in traditional understanding, medication habits and seed resources. Therefore, rapid and accurate identification methods for Bupleurum (Apiaceae) seeds are required. We analyzed the internal transcribed spacer (ITS) sequences of five common Bupleurum species to detect variations in them, including B. chinense, B. scorzonerifolium, B. marginatum var. stenophyllum, B. falcatum and B. smithii var.parvifolium. Based on single nucleotide polymorphisms (SNPs) in the ITS region, we designed five specific primer pairs and used these primers i

SUBMITTER: Qi W 

PROVIDER: S-EPMC7210332 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature