ATF2 and ATF7 Are Critical Mediators of Intestinal Epithelial Repair.

ABSTRACT:

Background & aims

Activation factor-1 transcription factor family members activating transcription factors 2 and 7 (ATF2 and ATF7) have highly redundant functions owing to highly homologous DNA binding sites. Their role in intestinal epithelial homeostasis and repair is unknown. Here, we assessed the role of these proteins in these conditions in an intestine-specific mouse model.

Methods

We performed in vivo and ex vivo experiments using Villin-Cre^ERT2Atf2^fl/flAtf7^ko/ko mice. We investigated the effects of intestinal epithelium-specific deletion of the Atf2 DNA binding region in Atf7^-/- mice on cellular proliferation, differentiation, apoptosis, and epithelial barrier function under homeostatic conditions. Subsequently, we exposed

SUBMITTER: Meijer BJ 

PROVIDER: S-EPMC7210476 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature