Cloning, sequencing, and expression analysis of 32 NAC transcription factors (MdNAC) in apple.

ABSTRACT:

Background

NAC transcription factors play important roles in the regulation of plant growth, development, abiotic and biotic stress responses. The transcriptional level of MdNACs in different tissues and under various biotic and abiotic stress treatments was determined to provide a solid foundation for studying the function of MdNACs.

Methods

Thirty-two full-length cDNA sequences of Md NACs were isolated by homologous comparison and RT-PCR confirmation, and the obtained cDNA sequences and the deduced amino acid sequences were analyzed with bioinformatics methods. The prediction of subcellular locations of MdNAC proteins was performed using CELLO v.2.5, PSORT, and SoftBerry ProtComp 9.0. Expression levels of MdNACs were detected in 16 different tis

SUBMITTER: Li H 

PROVIDER: S-EPMC7210808 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature