Evaluation of thrombospondin-1 gene polymorphisms in corneal allograft rejection in Asian Indian patients.

ABSTRACT:

Purpose

To evaluate the frequency and the association of Thrombospondin 1 (THBS1) gene single nucleotide polymorphisms (SNPs) in Asian Indian patients with optical full thickness corneal grafting surgery.

Methods

Prospective case-control analysis of optical penetrating keratoplasty patients with and without immune rejection and controls for genotyping of 3 THBS1 gene SNPs (rs1478604 A>G; rs2228261 C>T; rs2228262 A>G) by Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS PCR).

Results

Among 58 patients [45 with immune allograft rejection (DNA isolation was possible in 38 samples) and 13 without immune corneal allograft rejection] and 65 controls, allele frequencies observed for rs1478604 (A>G) are A: 69.7% and 72.6%, G: 30.2% and 27.3%; for rs2228

SUBMITTER: Vanathi M 

PROVIDER: S-EPMC7210848 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature