Biochemical characteristics of the chondrocyte-enriched SNORC protein and its transcriptional regulation by SOX9.

ABSTRACT: Snorc (Small NOvel Rich in Cartilage) has been identified as a chondrocyte-specific gene in the mouse. Yet little is known about the SNORC protein biochemical properties, and mechanistically how the gene is regulated transcriptionally in a tissue-specific manner. The goals of the present study were to shed light on those important aspects. The chondrocyte nature of Snorc expression was confirmed in mouse and rat tissues, in differentiated (day 7) ATDC5, and in RCS cells where it was constitutive. Topological mapping and biochemical analysis brought experimental evidences that SNORC is a type I protein carrying a chondroitin sulfate (CS) attached to serine 44. The anomalous migration of SNORC on SDS-PAGE was due to its primary polypeptide features, suggesting no additional post-translationa

SUBMITTER: Jaiswal PK 

PROVIDER: S-EPMC7210984 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature