Differential expression of the five redox complexes in the retinal mitochondria or rod outer segment disks is consistent with their different functionality.

ABSTRACT:

Purpose

The retinal rod outer segment (OS) disk membranes, devoid of mitochondria, conducts oxidative phosphorylation (OxPhos). This study aimed at identifying which proteins expressed in the retinal rod OS disks determined the considerable adenosine-5'-triphosphate production and oxygen consumption observed in comparison with retinal mitochondria.

Procedures

Characterization was conducted by immunogold transmission electron microscopy on retinal sections. OxPhos was studied by oximetry and luminometry. The proteomes of OS disks and mitochondria purified from bovine retinas were studied by mass spectrometry. Statistical and bioinformatic analyses were conducted by univariate, multivariate, and machine learning methods.

Results

Weighted gene coexpression network analys

SUBMITTER: Bruschi M 

PROVIDER: S-EPMC7211042 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature