Human mast cells exhibit an individualized pattern of antimicrobial responses.

ABSTRACT:

Introduction

Mast cells (MCs) are tissue-resident immune cells implicated in antibacterial responses. These include chemokine secretion, degranulation, and the release of mast cell-extracellular traps, which are primarily dependent on reactive oxygen species (ROS) production. Our study investigated whether human mast cells (hMCs) develop individual response patterns to bacteria located at different tissue sites: Escherichia coli (gut commensal), Listeria monocytogenes (foodborne intracellular pathogen), Staphylococcus aureus (skin commensal and opportunistic pathogen), and Streptococcus pneumoniae (upper respiratory tract commensal and lung pathogen).

Methods

After live bacteria exposure, hMCs were analyzed by a combined flow cytometry assay for degranulation, ROS production

SUBMITTER: Garcia-Rodriguez KM 

PROVIDER: S-EPMC7212193 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature