Detection of amastigotes and histopathological alterations in the thymus of Leishmania infantum-infected dogs.

ABSTRACT:

Introduction

In canine visceral leishmaniasis (CVL), lymphopenia, and the disorganization of lymphoid organs such as spleen and lymph nodes have been demonstrated. However, the involvement of thymus in CVL has not been evaluated so far. Herein, we investigated whether the thymus can be colonized by Leishmania infantum in naturally infected dogs.

Methods

Thymus were obtained from 16 of 58 dogs and samples of this organ were submitted to immunohistochemistry for laminin and fibronectin detection, histopathology, in situ hybridization and polymerase chain reaction (PCR) targeting the gene ITS-1 for Leishmania and sequenced. Samples of spleen, skin and popliteal lymph nodes were collected and submitted to immunohistochemistry and parasitological culture followed by multilocus en

SUBMITTER: da Silva AVA 

PROVIDER: S-EPMC7212199 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature