Changes in passively-sensitized basophil activation to αS1-casein after oral immunotherapy.

ABSTRACT:

Introduction

Immune response to cow's milk allergen (CMA) has been analyzed mostly using crude milk antigen or a mixture of various caseins. This study aimed to assess the changes in the immunological response against αS1-casein during oral immunotherapy (OIT) and to investigate the mechanism of tolerance.

Methods

We have performed rush OIT to 39 patients with CMA and obtained the serum samples up to 3 years after OIT. Immunoglobulin E (IgE) and IgG4 antibodies specific to highly purified αS1-casein as well as passively-sensitized basophil activation were evaluated using the serial samples. Furthermore, we examined whether basophil activation led by the pre-OIT serum was suppressed by the post-OIT serum, or by the tolerant serum obtained from naturally outgrown patients.

SUBMITTER: Matsui T 

PROVIDER: S-EPMC7212200 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature