Correlation between sperm mitochondrial ND5 and ND6 gene variations and total fertilisation failure.

ABSTRACT:

Introduction

The purpose of this study was to investigate the correlation between sperm mitochondrial NADH dehydrogenase subunit 5 (ND5) and NADH dehydrogenase subunit 6 (ND6) gene variations and total fertilisation failure (TFF).

Material and methods

A total of 232 sperm samples at the fresh in vitro fertilisation (IVF) cycle or the half-intracytoplasmic sperm injection (ICSI) cycle were collected for this retrospective controlled study on Han Chinese people between July 2011 and April 2014. Of the 232 total samples, 45 were from the IVF-TFF group and 187 were from couples with normal fertilisation (fertilisation rate > 50%). The mitochondrial ND5 and ND6 gene variations and sperm haplotypes were confirmed using nested PCR and DNA sequencing.

Results

Ten homozygous v

SUBMITTER: Mao GH 

PROVIDER: S-EPMC7212212 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature