Insights gained from a comprehensive all-against-all transcription factor binding motif benchmarking study.

ABSTRACT:

Background

Positional weight matrix (PWM) is a de facto standard model to describe transcription factor (TF) DNA binding specificities. PWMs inferred from in vivo or in vitro data are stored in many databases and used in a plethora of biological applications. This calls for comprehensive benchmarking of public PWM models with large experimental reference sets.

Results

Here we report results from all-against-all benchmarking of PWM models for DNA binding sites of human TFs on a large compilation of in vitro (HT-SELEX, PBM) and in vivo (ChIP-seq) binding data. We observe that the best performing PWM for a given TF often belongs to another TF, usually from the same family. Occasionally, binding specificity is correlated with the structural class of the DNA binding domain, indic

SUBMITTER: Ambrosini G 

PROVIDER: S-EPMC7212583 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature