Different p53 genotypes regulating different phosphorylation sites and subcellular location of CDC25C associated with the formation of polyploid giant cancer cells.

ABSTRACT:

Background

Our previous studies have confirmed that cobalt chloride (CoCl₂) can induce the formation of polyploid giant cancer cells (PGCCs), which is the key to the heterogeneity of solid tumors. PGCC formation is closely related to the abnormal expression of cell cycle-related proteins and cell fusion. In this study, we investigated the molecular mechanism of PGCCs formation by detecting the expression of cell cycle-related proteins in mutant and wild-type p53 cancer cell lines.

Methods

HEY, BT-549, SKOv3 and MDA-MB-231 cells were treated with CoCl₂ and the cell cycle was detected by flow cytometry. The expression and subcellular localization of cell cycle-related proteins, kinases, and P53 were compared before and after CoCl₂ treatment. Im

SUBMITTER: Liu K 

PROVIDER: S-EPMC7212590 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature