Functional Study of the Retrotransposon-Derived Human PEG10 Protease.

ABSTRACT: Paternally expressed gene 10 (PEG10) is a human retrotransposon-derived imprinted gene. The mRNA of PEG10 encodes two protein isoforms: the Gag-like protein (RF1_PEG10) is coded by reading frame 1, while the Gag-Pol-like polyprotein (RF1/RF2_PEG10) is coded by reading frames 1 and 2. The proteins are translated by a typical retroviral frameshift mechanism. The protease (PR) domain of RF2_PEG10 contains an -Asp-Ser-Gly- sequence, which corresponds to the consensus -Asp-Ser/Thr-Gly- active-site motif of retroviral aspartic proteases. The function of the aspartic protease domain of RF2_PEG10 remains unclear. To elucidate the function of PEG10 protease (PR_PEG10), we designed a frameshift mutant (_fsRF1/RF2_PEG10) for

SUBMITTER: Golda M 

PROVIDER: S-EPMC7212762 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature