Effects of valproic acid on histone deacetylase inhibition in vitro and in glioblastoma patient samples.

ABSTRACT:

Background

The antiepileptic drug valproic acid (VPA) inhibits histone deacetylase in glioblastoma cells in vitro, which influences several oncogenic pathways and decreases glioma cell proliferation. The clinical relevance of these observations remains unclear, as VPA does not seem to affect glioblastoma patient survival. In this study, we analyzed whether the in vitro effects of VPA treatment on histone acetylation are also observed in tumor tissues of glioblastoma patients.

Methods

The in vitro effects of VPA treatment on histone acetylation were assessed with immunofluorescence and western blotting. On tissue microarrays and in fresh-frozen glioblastoma tissues we investigated the histone acetylation patterns of patients who were either treated with VPA or did not receive

SUBMITTER: Berendsen S 

PROVIDER: S-EPMC7212905 | biostudies-literature | 2019 May-Dec

REPOSITORIES: biostudies-literature