Seminal plasma and CD4⁺ T-cell cytokine profiles in the in vitro fertilization success.

ABSTRACT:

Background

Abnormal female immune response is one of the potential causes of unexplained infertility (UI). Seminal plasma (SP) is an important regulator of female immune responses during pregnancy. This study investigated a SP effect on the expression of CD4⁺ T-cell-related cytokines in a group of UI woman candidates for in vitro fertilization (IVF) and healthy fertile women.

Materials and methods

This was a semi-experimental study that performed on 20 UI couples (ten unsuccessful and ten successful IVF outcomes) and 10 fertile couples as the healthy group. CD4⁺ T-cells were separated from peripheral blood mononuclear cells of women by magnetic-activated cell sorting technique and incubated with (stimulated condition) or without (unstimulated con

SUBMITTER: Kanannejad Z 

PROVIDER: S-EPMC7212999 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature