Growth and differentiation factor 15 is secreted by skeletal muscle during exercise and promotes lipolysis in humans.

ABSTRACT: We hypothesized that skeletal muscle contraction produces a cellular stress signal, triggering adipose tissue lipolysis to sustain fuel availability during exercise. The present study aimed at identifying exercise-regulated myokines, also known as exerkines, able to promote lipolysis. Human primary myotubes from lean healthy volunteers were submitted to electrical pulse stimulation (EPS) to mimic either acute intense or chronic moderate exercise. Conditioned media (CM) experiments with human adipocytes were performed. CM and human plasma samples were analyzed using unbiased proteomic screening and/or ELISA. Real-time qPCR was performed in cultured myotubes and muscle biopsy samples. CM from both acute intense and chronic moderate exercise increased basal lipolysis in human adipocytes. Grow

SUBMITTER: Laurens C 

PROVIDER: S-EPMC7213799 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature