NUP210 and MicroRNA-22 Modulate Fas to Elicit HeLa Cell Cycle Arrest.

ABSTRACT:

Purpose

Cervical cancer is one of the most fatal diseases among women in under-developed countries. To improve cervical cancer treatment, discovery of new targets is needed. In this study, we investigated the expression of NUP210, miR-22, and Fas in cervical cancer tissues and their functions in cell cycle regulation.

Materials and methods

We detected and compared the expression levels of NUP210, miR-22, and Fas in cervical cancer tissues with paired normal tissues using immunohistochemistry, Western blot, and real-time quantitative polymerase chain reaction. NUP210 was knocked down in HeLa cells via lentivirus, followed by cell cycle and proliferation analysis. Using a luciferase reporter assay, we explored the link between miR-22 and NUP210. We overexpressed miR-22 in HeLa

SUBMITTER: Gu Q 

PROVIDER: S-EPMC7214106 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature