Anti-inflammatory effects of naproxen sodium on human osteoarthritis synovial fluid immune cells.

ABSTRACT:

Objective

To evaluate the anti-inflammatory effects of clinically relevant naproxen sodium (Nx) concentrations on human monocyte-derived macrophages in a controlled in vitro system and human primary synovial fluid (SF) cells.

Design

Using phorbol 12-myristate 13-acetate, THP-1 human monocytic cells were differentiated into mature monocyte-derived macrophages in vitro then treated with Nx pre- or post-activating an inflammatory response with lipopolysaccharide (LPS) and hyaluronan (HA) fragments (n = 8/group). Cell culture supernatants were assessed for NF-κB activity and prostaglandin E₂ (PGE₂), indicating cyclooxygenase enzyme activity. Under Duke IRB approval, primary human SF cells were collected at the time of knee joint replacement (n = 19 individu

SUBMITTER: Hsueh MF 

PROVIDER: S-EPMC7214189 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature