MfOfd1 is crucial for stress responses and virulence in the peach brown rot fungus Monilinia fructicola.

ABSTRACT: Monilinia fructicola is the most widely distributed species among the Monilinia genus in the world, and causes blossom blight, twig canker, and fruit rot on Rosaceae fruits. To date, studies on genomics and pathogenicity are limited in M. fructicola. In this study, we identified a redox-related gene, MfOfd1, which was significantly up-regulated at 1 hr after inoculation of M. fructicola on peach fruits. We used the clustered regulatory inter-spaced short palindromic repeats (CRISPR)/Cas9 system combined with homologous recombination to determine the function of the MfOfd1 gene. The results showed that the sporulation of knockdown transformants was reduced by 53% to 83%. The knockdown transformants showed increased sensitivity to H₂ O₂ and decreased virulence on peach

SUBMITTER: Zhang MM 

PROVIDER: S-EPMC7214477 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature