Validated spectrophotometric method for quantification of total triterpenes in plant matrices.

ABSTRACT:

Background

Triterpenes are ubiquitous secondary metabolites present in plants. They can be found in both forms, as genins or conjugated as glycosides. Although distinct analytical methods to quantify these compounds in vegetal tissues are available in the literature, limitations like high cost, complexity on sample preparation, and selectivity are often challenging issues. This study aimed to develop and to validate a simple and rapid spectrophotometric method to detect and quantify total triterpenes in plant matrices.

Methods

The assay was conducted directly into glass tubes using vanillin, acetic acid, and sulphuric acid as reagents, and β-sitosterol as reference standard. The samples were analyzed at 548 nm assessing the quality parameters of selectivity, linearity, preci

SUBMITTER: Pedrosa AM 

PROVIDER: S-EPMC7214563 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature