Robot Cookies - Plant Cell Packs as an Automated High-Throughput Screening Platform Based on Transient Expression.

ABSTRACT: The high-throughput screening of recombinant protein expression is advantageous during early process development because it allows the identification of optimal expression constructs and process conditions. Simple screening platforms based on microtiter plates are available for microbes and animal cells, but this was not possible for plants until the development of plant cell packs (PCPs), also known as "cookies," which provide a versatile and scalable screening tool for recombinant protein production. PCPs are prepared from plant cell suspension cultures by removing the medium and molding the biomass. PCPs can be cast into 96-well plates for high-throughput screening, but the manual handling effort currently limits the throughput to ∼500 samples per day. We have therefore integrated the P

SUBMITTER: Gengenbach BB 

PROVIDER: S-EPMC7214789 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature