Estrogen regulates the proliferation and inflammatory expression of primary stromal cell in benign prostatic hyperplasia.

ABSTRACT:

Background

To investigate the expression of estrogen receptor (ER) in prostate tissues of benign prostatic hyperplasia (BPH) individuals, and the effects of estrogen regulating the proliferation and inflammatory expressions of primary prostate stromal cells in BPH.

Methods

A total of 44 human BPH prostate tissues were collected to explore the expression of ER by immunohistochemistry (IHC). Cell proliferation, mRNA and protein expressions were analyzed in primary prostate stromal cells treated with estrogen or estrogen plus fulvestrant through cell count kit-8 (CCK-8) assay, quantitative real-time polymerase chain reaction (qPCR), IHC and western blot, respectively.

Results

Firstly, ERβ was positive, and ERα was negative in the transition zone of prostate among all the

SUBMITTER: Chen B 

PROVIDER: S-EPMC7214965 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature