Sirt6 in pro-opiomelanocortin neurons controls energy metabolism by modulating leptin signaling.

ABSTRACT:

Objective

Sirt6 is an essential regulator of energy metabolism in multiple peripheral tissues. However, the direct role of Sirt6 in the hypothalamus, specifically pro-opiomelanocortin (POMC) neurons, controlling energy balance has not been established. Here, we aimed to determine the role of Sirt6 in hypothalamic POMC neurons in the regulation of energy balance and the underlying mechanisms.

Methods

For overexpression studies, the hypothalamic arcuate nucleus (ARC) of diet-induced obese mice was targeted bilaterally and adenovirus was delivered by using stereotaxic apparatus. For knockout studies, the POMC neuron-specific Sirt6 knockout mice (PKO mice) were generated. Mice were fed with chow diet or high-fat diet, and body weight and food intake were monitored. Whole-body en

SUBMITTER: Tang Q 

PROVIDER: S-EPMC7215198 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature