The Separation and Characterization of Extracellular Vesicles from Medium Conditioned by Bovine Embryos.

ABSTRACT: Extracellular vesicles (EVs) have been identified as one of the communication mechanisms amongst embryos. They are secreted into the embryo culture medium and, as such, represent a source of novel biomarkers for identifying the quality of cells and embryos. However, only small amounts of embryo-conditioned medium are available, which represents a challenge for EV enrichment. Our aim is to assess the suitability of different EV separation methods to retrieve EVs with high specificity and sufficient efficiency. Bovine embryo-conditioned medium was subjected to differential ultracentrifugation (DU), OptiPrepTM density gradient (ODG) centrifugation, and size exclusion chromatography. Separated EVs were characterized by complementary characterization methods, including Western blot, electron mi

SUBMITTER: Pavani KC 

PROVIDER: S-EPMC7215575 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature